ELISA or Enzyme-Linked Immunosorbant Assay is a commonly used immunoassay for detecting soluble analytes such as proteins, small molecules and peptides. This video focuses on ELISA troubleshooting strategies and is designed for both ready-to-use ELISA Kits and DIY (do it yourself) formats. Learn to manage and avoid unexpected pitfalls that can stand in your way of a great outcome. The troubleshooting tips are applicable to various sample types including serum, plasma, urine and other fluids, tissue homogenates, and cell culture supernatants. Whether you are a beginner or an experienced user or using any ELISA design such as Sandwich or Competitive, this brief tutorial is a springboard for preventing, troubleshooting and resolving commonly encountered situations.